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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Oct 4, 2025

Two-Dimensional Super-Resolution Visualization of Rat Brain Microvasculature Using Ultrasound Localization Microscopy

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Ultrasound super-resolution imaging with a hierarchical Kalman tracker.

Iman Taghavi¹, Sofie Bech Andersen², Carlos Armando Villagómez Hoyos³

¹Center for Fast Ultrasound Imaging, Department of Health Technology, Technical University of Denmark, DK 2800, Kgs. Lyngby Denmark.

|February 12, 2022

Summary

A new hierarchical Kalman (HK) tracker improves microbubble (MB) tracking for ultrasound super-resolution imaging (SRI). This advanced method enhances velocity estimation and vessel diameter accuracy, especially in challenging conditions with high MB concentrations and localization uncertainty.

Failed At:

2026-06-19T13:48:39.254593+00:00

Keywords:

Contrast-enhanced ultrasound (CEUS)Microbubble tracking Microscopy Super-resolution imaging (SRI)Ultrasound localization microscopy (ULM)

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