Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Experimental IgA nephropathy in mice.

M Sato, T Ideura, S Koshikawa

    Laboratory Investigation; a Journal of Technical Methods and Pathology
    |April 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Topography of cat medullary ventral surface hypoxic acidification.

    Journal of applied physiology (Bethesda, Md. : 1985)·1992
    Same author

    Ion channels on synaptic vesicle membranes studied by planar lipid bilayer method.

    Biophysical journal·1992
    Same author

    Nucleotide sequence of cDNA for porcine heme oxygenase and its expression in Escherichia coli.

    Biochemistry international·1992
    Same author

    Immunohistochemical evidence that rat FSH cells contain beta II-subspecies of protein kinase C.

    Endocrinologia japonica·1992
    Same author

    Postnatal ontogenesis of neurons containing GABAA alpha 1 subunit mRNA in the rat forebrain.

    Brain research. Molecular brain research·1992
    Same author

    Immunocytochemical and in situ hybridization evidence for the coexistence of GABA and tyrosine hydroxylase in the rat locus ceruleus.

    The Anatomical record·1992

    Researchers induced experimental IgA nephropathy in mice by blocking the reticuloendothelial system (RES) and administering a food antigen. This model closely mimics human IgA nephropathy, aiding further research.

    Area of Science:

    • Nephrology
    • Immunology
    • Experimental Pathology

    Background:

    • Immunological abnormalities in human IgA nephropathy suggest a potential role for food antigens and immune system dysfunction.
    • The reticuloendiculoendothelial system (RES) plays a crucial role in immune regulation and antigen clearance.

    Purpose of the Study:

    • To develop an experimental mouse model of IgA nephropathy.
    • To investigate the role of reticuloendiculoendothelial system (RES) dysfunction in the pathogenesis of IgA nephropathy.

    Main Methods:

    • Mice were orally administered lactalbumin (food antigen) and/or treated with colloidal carbon to block the RES.
    • Renal biopsies and histopathological observations were conducted at 18 and 30 weeks.
    • Electron microscopy and serological analysis were used to assess IgA deposition and serum IgA levels.

    Related Experiment Videos

    Main Results:

    • IgA deposition in the mesangial area was significantly higher in mice with RES blockage and lactalbumin administration (Group 2) compared to controls.
    • Histopathological analysis revealed lesions similar to human IgA nephropathy, including increased mesangial dense deposits, particularly in Group 2.
    • Elevated serum IgA levels in Group 2 correlated with the intensity of mesangial IgA deposition.

    Conclusions:

    • Experimental IgA nephropathy, mimicking human disease, can be induced in mice by combining continuous oral immunization with RES dysfunction.
    • This mouse model provides a valuable tool for studying the mechanisms and potential treatments for IgA nephropathy.