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Updated: Aug 8, 2026

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
07:24

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Published on: February 6, 2021

Etiology and diagnosis of bacterial postoperative endophthalmitis

R K Forster

    Ophthalmology
    |April 1, 1978
    PubMed

    Insights

    Determining the cause of postoperative endophthalmitis is crucial. Cultures of intraocular contents from 58 eyes revealed infectious etiology in 50%, with vitreous samples proving most effective for diagnosis.

    Area of Science:

    • Ophthalmology
    • Microbiology

    Background:

    • Postoperative endophthalmitis requires differentiation between infectious and sterile causes.
    • Accurate diagnosis is essential for appropriate treatment and visual outcome.

    Purpose of the Study:

    • To evaluate the diagnostic yield of culturing intraocular specimens in suspected postoperative endophthalmitis.
    • To compare the sensitivity of vitreous versus anterior chamber aspirates.
    • To assess the utility of membrane filtration for vitreous samples.

    Main Methods:

    • Culture of intraocular contents (vitreous and anterior chamber aspirates) from 58 eyes with suspected postoperative endophthalmitis.
    • Comparison of culture results from vitreous and anterior chamber samples.
    • Analysis of stained smears and use of membrane filtration for vitreous samples.

    Main Results:

    • Intraocular cultures were positive in 50% of the tested eyes.
    • Vitreous aspirates demonstrated higher sensitivity for diagnosis compared to anterior chamber aspirates.
    • Membrane filtration of vitreous samples increased diagnostic yield.
    • Gram-positive organisms were identified in 50% of culture-positive cases.
    • Stained smears correlated with culture results in 50% of cases.

    Conclusions:

    • Infectious etiology is common in postoperative endophthalmitis.
    • Vitreous sampling, especially with membrane filtration, is superior for microbiological diagnosis.
    • Microbiological confirmation aids in guiding treatment for endophthalmitis.

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