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Serotype Identification of Human Adenoviruses Associated with Influenza-Like Illnesses in the Philippines from
Catherine Calzado-Dacasin1, Janiza Lianne Foronda2, Vina Lea Arguelles2
1Department of Virology, Research Institute for Tropical Medicine, Muntinlupa, Philippines; Graduate School, University of Santo Tomas, Manila, Philippines.
Objectives:
Human adenoviruses (HAdV) are known to cause a wide range of diseases including acute respiratory infections, conjunctivitis, and acute gastroenteritis. In this study, we aimed to determine the serotypes of HAdV in patients with influenza-like illness (ILI) in the Philippines from 2006-2012 and to describe the demographic and epidemiological characteristics of patients who tested positive for HAdV.
Methods:
Between 2006 and 2012, the Philippine National Influenza Centre detected HAdV in 1294 samples of patients with ILI. Serotype determination was done in select samples using microneutralization, polymerase chain reaction (PCR), and sequencing methods.
Results:
A total of 8 serotypes were identified (HAdV 1-7 and 11), with HAdV-2 (27.8%), and HAdV-3 (27.8%) being the most prevalent. The majority of HAdV infections were found in children below 5 years of age (79.9%).
Conclusions:
The identification of HAdV circulating serotypes may serve as guide for designing disease intervention and control strategies and will provide important information regarding the contribution of this virus to respiratory infections, particularly in children, which remain a public health burden in the Philippines.
Insights
Human adenoviruses (HAdV) caused influenza-like illness in Philippine children under five. HAdV-2 and HAdV-3 were the most common types identified between 2006-2012.
Area of Science:
- Virology
- Public Health
- Epidemiology
Background:
- Human adenoviruses (HAdV) are significant human pathogens responsible for diverse illnesses, including respiratory infections, conjunctivitis, and gastroenteritis.
- Understanding the circulating HAdV serotypes is crucial for public health interventions, especially in regions with a high burden of respiratory diseases.
Purpose of the Study:
- To identify the specific serotypes of Human Adenoviruses (HAdV) circulating among patients presenting with influenza-like illness (ILI) in the Philippines.
- To characterize the demographic and epidemiological features of individuals diagnosed with HAdV infection during the study period (2006-2012).
Main Methods:
- Analysis of 1294 samples from patients with ILI collected between 2006 and 2012 by the Philippine National Influenza Centre.
- Serotype determination of detected HAdV strains utilizing microneutralization assays, polymerase chain reaction (PCR), and DNA sequencing.
Main Results:
- Eight distinct HAdV serotypes were identified: HAdV types 1 through 7, and type 11.
- Human Adenovirus type 2 (HAdV-2) and Human Adenovirus type 3 (HAdV-3) were the most frequently detected serotypes, each accounting for 27.8% of positive cases.
- A significant majority of HAdV infections, 79.9%, occurred in children under the age of five.
Conclusions:
- The identified circulating HAdV serotypes provide essential data for developing targeted disease control and intervention strategies in the Philippines.
- This study highlights the substantial contribution of HAdV to the burden of respiratory infections in children, underscoring its importance as a public health concern.
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