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PtrHAT22, as a higher hierarchy regulator, coordinately regulates secondary cell wall component biosynthesis in
Mengxuan Ren1, Yang Zhang2, Ruiqi Wang3
1Research Center of Saline and Alkali Land of State Forestry and Grassland Administration, Chinese Academy of Forestry, Beijing, 100091, PR China.
Plant Science : an International Journal of Experimental Plant Biology
|February 13, 2022
Summary
Homeodomain-leucine zipper II transcription factors regulate plant growth and secondary cell wall composition. PtrHAT22 represses key genes, impacting lignin and cellulose in poplar.
Area of Science:
- Plant Biology
- Molecular Biology
- Forestry
Background:
- Homeodomain-leucine zipper (HD-Zip) II transcription factors (TFs) are crucial for plant development.
- The role of HD-Zip II TFs in regulating secondary cell wall (SCW) formation in woody plants is not well understood.
Purpose of the Study:
- To functionally characterize the Populus trichocarpa HD-Zip II TF, PtrHAT22, and investigate its role in SCW biosynthesis.
- To elucidate the regulatory mechanism of PtrHAT22 in poplar SCW formation.
Main Methods:
- Functional characterization of PtrHAT22 in transgenic poplar.
- Analysis of plant growth parameters, SCW composition (lignin, cellulose, hemicellulose), and cell wall thickness.
- Gene expression analysis of wood-associated TFs and structural genes.
- Promoter activity assays and cis-acting element binding analysis.
Main Results:
- Overexpression of PtrHAT22 resulted in arrested growth, reduced biomass, and altered SCW composition, with decreased lignin and cellulose but increased hemicellulose.
- PtrHAT22 acts as a transcriptional repressor, directly inhibiting the promoter activity of PtrMYB20, PtrMYB28, and PtrCOMT2.
- Expression of wood-associated TFs and structural genes was significantly altered in PtrHAT22 transgenic poplar.
Conclusions:
- PtrHAT22 plays a significant role in coordinating poplar SCW component biosynthesis.
- PtrHAT22 regulates SCW formation through both direct and indirect mechanisms, involving structural genes and other TFs in the SCW network.
- PtrHAT22 functions as a higher-hierarchy TF in the regulation of poplar SCW formation.
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