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Using feeding and motility patterns for ivermectin resistance detecting in Haemonchus contortus larvae
Yang Liu1, Xiaoping Luo2, Junyan Li2
1College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, Inner Mongolia, China; School of Life Sciences, Ningxia University, Yinchuan, 750021, Ningxia, China.
Experimental Parasitology
|February 13, 2022
Summary
Ivermectin (IVM) resistance in Haemonchus contortus is a global issue. Larval migration and feeding inhibition tests effectively identified IVM-resistant strains, aiding in parasite management.
Area of Science:
- Veterinary Parasitology
- Anthelmintic Resistance
- Molecular Biology
Background:
- Ivermectin resistance in Haemonchus contortus poses a significant global challenge.
- Accurate drug resistance testing is crucial for effective parasite control and monitoring anthelmintic efficacy.
Purpose of the Study:
- To evaluate and compare the ivermectin resistance of seven Haemonchus contortus strains using two distinct bioassays.
- To determine the reliability of larval migration inhibition tests (LMIT) and larval feeding inhibition tests (LFIT) for assessing ivermectin resistance.
Main Methods:
- Utilized the larval migration inhibition test (LMIT) and larval feeding inhibition test (LFIT).
- Assessed seven Haemonchus contortus strains: one resistant (UKR) and one susceptible (ASS) from the UK, and five native Chinese strains (SXS, WMR, WSR1, WSR2, WSR3).
- Employed fluorescent-labeled Escherichia coli for LFIT and monitored larval migration for LMIT after ivermectin treatment.
Main Results:
- LFIT indicated ivermectin treatment allowed fluorescent Escherichia coli to be observed within larvae of UKR, WMR, WSR1, WSR2, and WSR3 strains, but not in ASS and SXS strains.
- LMIT showed no significant change in migration for the SXS strain post-treatment compared to the susceptible ASS strain.
- Significant increases in migration were observed for UKR, WMR, WSR1, WSR2, and WSR3 strains after ivermectin treatment.
Conclusions:
- The SXS strain was identified as ivermectin-susceptible, while WMR, WSR1, WSR2, and WSR3 strains exhibited ivermectin resistance.
- Larval motility and feeding ability assessments via LMIT and LFIT are effective methods for determining Haemonchus contortus resistance to ivermectin.
