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Related Experiment Video

Updated: Oct 3, 2025

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract
10:07

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

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Cervix chip mimicking cervical microenvironment for quantifying sperm locomotion.

Sai-Xi Yu1, Yanan Liu2, Yi Wu1

  • 1Shanghai Institute of Cardiovascular Diseases, Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Department of Systems Biology for Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Biosensors & Bioelectronics
|February 13, 2022
PubMed
Summary

A novel cervix chip using hyaluronic acid effectively filters sperm, identifying highly motile sperm crucial for fertilization. This technology offers a simple, efficient tool for diagnosing male infertility by assessing sperm selection in a simulated cervical environment.

Keywords:
Cervix chipHyaluronic acidMicrofluidicsSperm

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Area of Science:

  • Reproductive Biology
  • Biomicrofluidics
  • Sperm Physiology

Background:

  • The cervix and its mucus are critical for sperm transport into the female reproductive tract.
  • Male infertility can result from impaired sperm passage through the cervical microenvironment.
  • Understanding sperm behavior within the cervix is essential for diagnosing infertility.

Purpose of the Study:

  • To investigate sperm behavior and selection within a simulated cervical microenvironment.
  • To develop a microfluidic device (cervix chip) for studying sperm-cervix interactions.
  • To establish a tool for identifying sperm with high motility for potential infertility diagnosis.

Main Methods:

  • Utilized hyaluronic acid (HA) as a cervical mucus substitute to mimic the cervix microenvironment.
  • Designed and employed a specialized cervix chip to observe sperm accumulation and escape.
  • Analyzed sperm motility and kinematic features within the HA-filled chip.

Main Results:

  • Hyaluronic acid effectively accumulated sperm, acting as a reservoir similar to natural cervical mucus.
  • Sperm that successfully escaped the HA demonstrated significantly higher motility compared to those that entered.
  • The cervix chip demonstrated HA's function as a selective filter for highly active sperm.

Conclusions:

  • The developed cervix chip provides a practical platform for studying sperm-cervix microenvironment interactions.
  • This device enables on-demand sperm selection based on kinematic features.
  • The cervix chip offers a simple, fast, and efficient method for clinical infertility diagnosis.