Construction of ultrasmooth PTFE membrane for preventing bacterial adhesion and cholestasis

Bacterial adhesion and bile sludge accumulation can increase the risk of complications such as stent restenosis after biliary stent implantation. Compared with active and passive antimicrobial surfaces, a significant advantage of slippery liquid-infused porous surfaces (SLIPSs) is their recoverable anti-adhesive properties. According to the mechanism of SLIPSs and the application environments of the biliary system, a polytetrafluoroethylene (PTFE) electrospun fibrous membrane-impregnated silicone-oil system was developed to construct an ultrasmooth surface. Experimental results indicated that a PTFE SLIPS with 350 cSt of silicone oil had an extremely small roll angle (< 5°) and a high slip rate (4.8 ± 0.1 mm/s) and maintained excellent sliding stability after 7 d of immersion in model bile system. Thus, it can inhibit the adhesion of proteins, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and bile sludge. Moreover, when human fibroblasts were cultured on the PTFE SLIPS, it exhibited good cytocompatibility. Therefore, the proposed ultrasmooth PTFE membranes provide a promising alternative for biliary system to prevent bacterial adhesion and bile sludge accumulation.