Cationic inulin as a new surface decoration hydrocolloid for improving the stability of liposomal nanocarriers

The aim of the present study was to investigate the cationization of inulin with Williamson's etherification method, and compare cationic inulin with unmodified inulin coatings for stabilizing nanoliposomes (NLPs). The synthetized cationic inulin was characterized by Fourier transforms infrared (FT-IR) spectroscopy, carbon hydrogen nitrogen (CHN) elemental analysis, and energy-dispersive X-ray spectroscopy. Three concentrations of inulin and cationic inulin (1, 2, and 4 mg/mL) were used for the coating of NLPs. The concentration of 4 mg/mL was found to be optimal for inulin and cationic inulin as surface coating, on the basis of particle size, zeta potential, and microstructural morphology. The lowest values of particle size (93.41 nm), polydispersity index (0.25), and negative zeta potential (-24.41 mV) were related to the coated NLPs with cationic inulin at a concentration of 4 mg/mL. The transmission electron microscopy image of the coated NLPs with cationic inulin exhibited a spherical and core-shell structure. The coated NLPs with cationic inulin showed the highest thermal stability, physical stability, and oxidative stability. In conclusion, cationic inulin coating conferred a stronger protection than the unmodified inulin coating of NLPs. The technique developed here can be applied for surface decoration of NLPs to improve their stability.