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Updated: Oct 3, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
Involvement of miR-769-5p/Retinoic Acid Receptor Responder 1 Axis in the Progression of Osteosarcoma:
Lina Ma1, Yongsheng Ma2, Ailing Lian3
1Department of Outpatient Center Operating Room, First Affiliated Hospital of Harbin Medical University, Harbin, China.
Introduction:
This study aimed to detect the function of retinoic acid receptor responder 1 (RARRES1) and its regulator miR-769-5p in the growth and mobility of osteosarcoma cells.
Methods:
The Gene Expression Omnibus database was applied to analyze RARRES1 and miR-769-5p expression, and the survival rate of osteosarcoma patients. The target association between miR-769-5p and RARRES1 was speculated by miRWalk, TargetScan, and miRanda Web sites, as well as affirmed by dual luciferase assay. RARRES1 expression was tested by quantitative real-time polymerase chain reaction and Western blot. The malignant properties of MG-63 and U2OS cells were assessed by a series of biological experiments.
Results:
RARRES1 was lowly expressed in osteosarcoma patients, which resulted in unfavorable survival. Depletion of RARRES1 promoted the viability and mobility of osteosarcoma cells. Moreover, miR-769-5p was affirmed as an upstream regulator of RARRES1 and negatively regulated RARRES1 expression. miR-769-5p upregulation accelerated the viability and mobility of osteosarcoma cells, which can be blocked by RARRES1 overexpression. miR-769-5p inhibitor suppressed the effect of malignant viability and mobility of osteosarcoma cells, while this suppressive effect was abolished by depleting RARRES1.
Discussion/Conclusion:
miR-769-5p promoted cell viability, invasion, and migration by reducing RARRES1 expression in osteosarcoma cells, which might provide novel targets for the treatment of osteosarcoma.
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