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Enabling single-molecule localization microscopy in turbid food emulsions
Abbas Jabermoradi1, Suyeon Yang1, Martijn I Gobes1
1Laboratory of Biophysics, Wageningen University and Research, Stippeneng 4, Wageningen 6708, The Netherlands.
Summary
We enhanced food microscopy using adaptive optics (AO) and flat-field illumination to overcome turbidity. This allows for super-resolution imaging of food colloids like mayonnaise, revealing structures at the nanoscale.
Area of Science:
- Food science
- Microscopy
- Biophysics
Background:
- Turbidity in food samples significantly hinders microscopic analysis due to refractive index mismatches.
- Existing microscopy techniques struggle to achieve high resolution in opaque food systems.
Purpose of the Study:
- To improve microscopic characterization of turbid food systems by enhancing resolution and depth penetration.
- To develop and implement adaptive optics (AO) and flat-field illumination within an open microscopy framework (miCube).
Main Methods:
- Integrated AO with a deformable mirror to correct aberrations and engineer point spread functions (PSFs).
- Utilized PSF engineering for 3D single-molecule localization microscopy (SMLM).
- Modeled a mayonnaise-like oil-in-water emulsion with fluorescently labeled phosvitin from egg yolk.
Main Results:
- Achieved sub-100 nm resolution imaging in 2D and 3D of phosvitin-coated oil droplets.
- Demonstrated homogeneous distribution of phosvitin at the emulsion interface.
- Successfully compensated for optical aberrations in a turbid food model system.
Conclusions:
- The enhanced miCube framework with AO enables super-resolution imaging in turbid food emulsions.
- This technique allows for nanoscale localization of biomacromolecules at colloidal interfaces.
- Paves the way for advanced characterization of complex food structures.
