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At the molecular resolution with MINFLUX?

Kirti Prakash1,2,3

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PubMed
Summary

This review critically examines the 1-3 nm spatial resolution claims of MINFLUX super-resolution microscopy. It evaluates MINFLUX against other techniques, considering factors like detection efficiency and limitations in dense biological samples.

Keywords:
MINFLUXSMLMSTEDimage resolutionlocalization precisionsuper-resolution imaging

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Area of Science:

  • Biophysics
  • Microscopy
  • Cell Biology

Background:

  • MINFLUX (Minimal Photon Flux) microscopy is presented as a revolutionary technique.
  • It claims unprecedented spatial resolution of 1-3 nm in fixed and living cells.
  • This claim suggests the potential for true molecular resolution imaging.

Purpose of the Study:

  • To critically evaluate the claimed 1-3 nm spatial resolution of MINFLUX.
  • To compare MINFLUX performance with existing super-resolution microscopy methods.
  • To identify potential limitations of MINFLUX for biological imaging.

Main Methods:

  • Comparative analysis of MINFLUX with other super-resolution techniques.
  • Focus on spatial resolution claims, localization precision, and detection efficiency.
  • Evaluation of labeling efficiency and performance in densely labeled biological structures.

Main Results:

  • Concerns raised regarding the attainment of true 1 nm resolution by MINFLUX.
  • Analysis highlights subjective filtering of localizations as a potential issue.
  • Detection versus labeling efficiency is identified as a critical factor.

Conclusions:

  • The study provides parameters for evaluating single-molecule super-resolution techniques.
  • Findings are relevant for researchers, developers, and facility managers considering MINFLUX.
  • Further research is needed to fully understand MINFLUX capabilities and limitations.