Flow cytometry detection and quantification of CAR T cells into solid tumors

Area of Science:

• Immunology
• Oncology
• Biotechnology

Background:

• Adoptive T cell therapy (ACT) using chimeric antigen receptor (CAR) T cells has succeeded against B-cell malignancies.
• CAR T cell therapy faces challenges in treating solid tumors, particularly regarding effective tumor infiltration.
• Standardized methods are needed to assess CAR T cell trafficking and efficacy in solid tumors.

Purpose of the Study:

• To develop and describe a flow cytometry method for detecting and quantifying CAR T cells within solid tumors.
• To provide a standardized approach for evaluating CAR T cell infiltration in solid tumor models.
• To facilitate the comparison of novel strategies aimed at enhancing CAR T cell infiltration.

Main Methods:

• Development of a flow cytometry protocol for single-cell preparation from solid tumors.
• Intravenous injection of CAR T cells into tumor-bearing models.
• Quantification of CAR T cell presence within the tumor mass using flow cytometry.

Main Results:

• The described flow cytometry method allows for rapid detection of CAR T cells within solid tumors.
• The protocol enables quantification of CAR T cell infiltration following intravenous administration.
• This method provides a standardized tool for assessing CAR T cell trafficking.

Conclusions:

• A reliable flow cytometry method for measuring CAR T cell infiltration in solid tumors has been established.
• This technique is crucial for advancing CAR T cell therapy for solid malignancies.
• Standardized assessment of CAR T cell trafficking will accelerate the development of more effective cancer treatments.