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Related Experiment Video

Updated: Oct 3, 2025

Using Q Suture to Enhance Resistance to Gap Formation and Tensile Strength of Repaired Flexor Tendons
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Twin prime editor: seamless repair without damage.

Muhammad Jawad Akbar Awan1, Zahir Ali2, Imran Amin1

  • 1Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College of Pakistan Institute of Engineering and Applied Sciences, Jhang Road, Faisalabad, Pakistan.

Trends in Biotechnology
|February 14, 2022
PubMed
Summary

Twin prime editing offers a new way to precisely alter large DNA sequences. This advanced CRISPR-Cas9 tool avoids DNA double-strand breaks, overcoming limitations of previous gene-editing methods.

Keywords:
DNA deletionDNA replacementDSB-independent genome editingpegRNAprime editortwin prime editing

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR-Cas9 technology enables targeted genomic DNA modification.
  • CRISPR-Cas-mediated DNA double-strand breaks (DSBs) can lead to undesirable random insertions or deletions, limiting precision.
  • Existing gene editing tools face challenges in large-scale DNA sequence replacement, integration, or deletion.

Purpose of the Study:

  • To introduce a novel gene editing tool, 'twin prime editing', for precise large genomic DNA manipulation.
  • To overcome the limitations associated with DNA double-strand breaks in CRISPR-Cas technology.
  • To enable seamless replacement, integration, or deletion of substantial genomic DNA segments.

Main Methods:

  • Development of the 'twin prime editing' system by Anzalone et al.
  • Utilizing prime editing principles to facilitate targeted DNA sequence modifications.
  • Experimental validation of the tool's efficacy in modifying large genomic regions without inducing DSBs.

Main Results:

  • Successful replacement, integration, and deletion of large genomic DNA sequences were achieved.
  • The twin prime editing tool demonstrated high precision and efficiency.
  • The method effectively avoided the generation of DNA double-strand breaks, a significant improvement over standard CRISPR-Cas9.

Conclusions:

  • Twin prime editing represents a significant advancement in gene editing technology.
  • This novel tool offers enhanced precision and versatility for genomic DNA manipulation.
  • The avoidance of DSBs makes twin prime editing a safer and more effective approach for complex genetic modifications.