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Related Concept Videos

Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

415
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
415
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

584
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
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Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

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Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
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Key Techniques in Microbiology01:29

Key Techniques in Microbiology

704
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
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Methods for Controlling Microbial Growth01:29

Methods for Controlling Microbial Growth

683
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
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Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

291
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
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Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments.

Shanna-Leigh Davidson1, Tagbo H R Niepa1,2,3,4,5,6

  • 1Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, United States.

Frontiers in Microbiology
|February 14, 2022
PubMed
Summary

Micro-technologies enable high-throughput microbial culturing in controlled environments, aiding the discovery of novel species and metabolic pathways for applications like drug discovery and diagnostics.

Keywords:
microarraysmicrofluidicsmicromachined devicesnanoculturesunculturable microbes

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Area of Science:

  • Microbiology
  • Bioengineering
  • Biotechnology

Background:

  • Microbial communities are crucial in various biological processes, but many species remain
  • unculturable
  • using traditional methods.
  • Microfabrication advances enable miniaturized culturing techniques for precise control and analysis of microbial environments.

Purpose of the Study:

  • To review advancements in miniaturized microbial culturing techniques.
  • To highlight the potential of micro-technologies for discovering and cultivating previously unculturable microbes.
  • To discuss challenges hindering the scalability and commercialization of these technologies.

Main Methods:

  • Discussion of three key miniaturized culturing approaches: microarrays, micromachined devices, and microfluidics.
  • Analysis of the benefits offered by micro-technologies, including high-throughput screening, reduced resource use, and real-time optical access.
  • Examination of the current proof-of-concept status and limitations of micro-technologies.

Main Results:

  • Micro-technologies offer significant advantages for microbial research, including enhanced culturing capabilities and detailed observation.
  • These methods facilitate the exploration of novel metabolic pathways essential for drug discovery and diagnostics.
  • The potential exists to culture previously inaccessible microbial species.

Conclusions:

  • Miniaturized culturing techniques, including microarrays, micromachined devices, and microfluidics, represent a significant advancement in microbial research.
  • Overcoming challenges in accessibility, cost, and usability is crucial for the widespread adoption and commercialization of micro-technologies.
  • Further development will accelerate discoveries in microbiology, medicine, and biotechnology.