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Published on: April 19, 2015
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Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration
Simara Laboy-López1,2, Pedro O Méndez Fernández2,3, Jorge G Padilla-Zayas2,3
1Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, 17 University Ave. 1701, San Juan 00925, USA.
International Journal of Biomaterials
|February 14, 2022
Summary
Bioactive cellulose acetate fiber mats show potential for bone tissue engineering. Modified with peptides, these scaffolds enhance osteoblast adhesion and proliferation, aiding new bone formation.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Current cell-based bone tissue engineering (BTE) models struggle to replicate the bone microenvironment.
- Developing scaffolds that mimic the bone extracellular matrix (ECM) is crucial for bone tissue regeneration (BTR).
Purpose of the Study:
- To create and evaluate a porous cellulose acetate (CA) fiber mat scaffold for BTE.
- To bioactivate the CA scaffold surface to promote osteoconduction and osteoinduction.
Main Methods:
- Electrospinning was used to fabricate porous CA fiber mats.
- Chemical modifications and peptide coupling (KRSR, RGD, BMP-2) were performed for bioactivation.
- Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS) characterized the mats.
- Immunocytochemical (ICC) techniques assessed human fetal osteoblast (hFOB 1.19) adhesion, proliferation, and differentiation.
Main Results:
- FTIR and SEM/EDS confirmed successful chemical modifications and structural integrity.
- Peptide presence significantly enhanced hFOB 1.19 cell adhesion and proliferation at 48 hours and 7 days.
- No significant effect on osteogenic progenitor cell differentiation and maturation was observed at 14 days.
Conclusions:
- Porous CA fiber mats offer a biocompatible scaffold with promising surface morphology for BTE.
- Bioactivation with osteogenic peptides enhances osteoconduction, supporting new tissue formation.
