Related Experiment Video

Updated: Oct 3, 2025

Nerve Excitability Assessment in Chemotherapy-induced Neurotoxicity
07:42

Nerve Excitability Assessment in Chemotherapy-induced Neurotoxicity

Published on: April 26, 2012

18.3K

Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

Sandra Pampín-Suárez1, José Luis Arce-Diego1, Olga Tapia2

  • 1Biomedical Engineering Group, TEISA Department, University of Cantabria, Avenida de los Castros 46, 39005 Santander, Spain.

Biomedical Optics Express
|February 14, 2022
PubMed

Insights

Oxazine 4-perchlorate (O4P) shows promise for nerve identification in surgery. Concentrations below 100 µM are safe for U87 glioma cells, supporting its potential as a nerve biomarker.

Area of Science:

  • Biomedical optics
  • Surgical technology
  • Cellular toxicology

Background:

  • Accurate nerve identification is crucial in guided surgery to prevent collateral damage.
  • Fluorescence imaging using oxazine 4-perchlorate (O4P) is a potential method for nerve visualization.
  • Assessing the safety and efficacy of novel biomarkers is essential for clinical translation.

Purpose of the Study:

  • To evaluate the cytotoxicity of oxazine 4-perchlorate (O4P) on U87 human-derived glioma cells.
  • To determine the safety of O4P under various operating room irradiation conditions.
  • To investigate the potential of O4P as a nerve biomarker in surgical applications.

Main Methods:

  • Cytotoxicity assays were performed on U87 glioma cells exposed to varying concentrations of O4P.
  • Cells were subjected to different optical irradiation modes using a custom-built device for controlled dosimetry.
  • Immunofluorescence and confocal microscopy were utilized to assess DNA damage and O4P intracellular localization.

Main Results:

  • O4P exhibited no significant cytotoxicity on U87 glioma cells at concentrations below 100 µM.
  • Intracellular localization and DNA damage were analyzed under different irradiation conditions.
  • The study established a safe concentration threshold for O4P in a cellular model.

Conclusions:

  • Oxazine 4-perchlorate (O4P) demonstrates a favorable safety profile at concentrations below 100 µM for U87 glioma cells.
  • These findings support the feasibility of using O4P as a nerve biomarker in guided surgery.
  • Further research is warranted to validate O4P's efficacy and safety in vivo for surgical applications.

More Related Videos

In Vivo Electrophysiological Measurement of the Rat Ulnar Nerve with Axonal Excitability Testing04:56

In Vivo Electrophysiological Measurement of the Rat Ulnar Nerve with Axonal Excitability Testing

Published on: February 6, 2018

9.4K
Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depola10:45

Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depola

Published on: January 13, 2012

16.1K

Related Concept Videos

Local Anesthetics: Differential Sensitivity of Nerve Fibers01:24

Local Anesthetics: Differential Sensitivity of Nerve Fibers

Local anesthetics (LAs) block the sodium channels of nerve trunks, sensory nerve endings, and neuromuscular junctions. Although LAs can block all kinds of nerves, the sensitivity of nerve fibers differs according to nerve types and structures. LAs are known to block myelinated fibers faster than unmyelinated ones. Also, they block pain or sensory neurons at low concentrations without affecting the motor neurons involved in muscle contractions. This helps relieve labor pain without affecting the...
978