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Related Experiment Video

Updated: Oct 3, 2025

Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

Published on: August 23, 2022

F-CUBIC: a rapid optical clearing method optimized by quantitative evaluation.

Lina Liu^1,2,3, Xianyuan Xia^1,2,3, Feng Xiang^1,2

¹Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Biomedical Optics Express

|February 14, 2022

Summary

This summary is machine-generated.

Researchers developed F-CUBIC, a rapid optical clearing method for millimeter-thick tissues. This fast and versatile protocol enables deep-tissue imaging within minutes, offering potential for quick 3D biopsy and histopathological examinations.

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Last Updated: Oct 3, 2025

Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

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Published on: August 23, 2022

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

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Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Published on: July 22, 2019

Area of Science:

Biomedical Engineering
Optical Imaging
Tissue Clearing

Background:

Deep-tissue imaging is crucial for understanding biological processes but limited by light scattering in thick specimens.
Existing optical clearing methods often require long processing times or cause significant tissue distortion.
A need exists for rapid, safe, and efficient optical clearing protocols for millimeter-thick tissues.

Purpose of the Study:

To develop and validate a fast and versatile optical clearing protocol for millimeter-thick biological tissues.
To introduce a simple chemical screening method for evaluating optical clearing reagents.
To demonstrate the application of the novel protocol for non-destructive 3D biopsy.

Main Methods:

A chemical screening method using porcine small intestine tissue was established to quantify clearing reagent speed.
A new protocol, F-CUBIC (formamide-added CUBIC), was developed based on screening results.
F-CUBIC involves one-step immersion of millimeter-thick tissues at room temperature for 2-20 minutes.

Main Results:

F-CUBIC achieved rapid clearing of millimeter-thick tissues within 2-20 minutes.
The protocol demonstrated negligible tissue distortion and high compatibility with fluorescent labeling.
Successful application of F-CUBIC for non-destructive 3D biopsy of human colon specimens using two-photon microscopy was shown.

Conclusions:

F-CUBIC is a fast, economical, and versatile optical clearing protocol suitable for millimeter-thick tissues.
This method significantly reduces processing time compared to existing techniques.
F-CUBIC holds substantial potential for rapid 3D tissue mapping, aiding biomedical research and clinical applications like instant histopathological examinations.