Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

569
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
569
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

724
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
724
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

7.4K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
7.4K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

15.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
15.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sesamin attenuates atherosclerosis by alleviating vascular endothelial ferroptosis-related injury <i>via</i> m<sup>6</sup>A-dependent regulation of SREBF1 expression.

Frontiers in cell and developmental biology·2026
Same author

Identification of Dopamine D2 Receptor as a Direct Target of Salidroside and Tyrosol by Integrated Transcriptomic and Biophysical Approaches.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Deciphering Metabolic Alterations Associated with Glioma Grading Using Hyperspectral Stimulated Raman Scattering Imaging.

Analytical chemistry·2025
Same author

Co-blocking TIGIT and PVRIG Using a Novel Bispecific Antibody Enhances Antitumor Immunity.

Molecular cancer therapeutics·2025
Same author

Engineered Mycobacterium tuberculosis triple-kill-switch strain provides controlled tuberculosis infection in animal models.

Nature microbiology·2025
Same author

A BCG kill switch strain protects against Mycobacterium tuberculosis in mice and non-human primates with improved safety and immunogenicity.

Nature microbiology·2025

Related Experiment Video

Updated: Oct 3, 2025

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
07:05

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

Published on: June 20, 2025

1000

Optical coherence tomography-guided confocal Raman microspectroscopy for rapid measurements in tissues.

Xiaojing Ren1,2, Kan Lin3,2, Chao-Mao Hsieh1

  • 1School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, 637457, Singapore.

Biomedical Optics Express
|February 14, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a combined confocal Raman spectroscopy (CRS) and optical coherence tomography (OCT) system for rapid, depth-resolved tissue analysis. The system enables precise targeting for biochemical fingerprinting, aiding in disease diagnosis and margin assessment.

More Related Videos

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.2K
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.6K

Related Experiment Videos

Last Updated: Oct 3, 2025

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
07:05

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

Published on: June 20, 2025

1000
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.2K
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.6K

Area of Science:

  • Biomedical optics
  • Spectroscopy
  • Medical imaging

Background:

  • Accurate tissue characterization is crucial for disease diagnosis.
  • Confocal Raman spectroscopy (CRS) provides biochemical information.
  • Optical coherence tomography (OCT) offers high-resolution morphological imaging.

Purpose of the Study:

  • To develop and demonstrate a hybrid CRS-OCT system.
  • To enable rapid, depth-specific Raman measurements guided by OCT.
  • To facilitate targeted biochemical analysis of biological tissues.

Main Methods:

  • Integration of CRS and OCT modules.
  • Utilization of an electrically tunable lens for rapid depth focusing in CRS.
  • Depth-dependent measurements on ex vivo swine tissue and in vivo human skin.

Main Results:

  • The joint system achieved rapid targeting of regions of interest.
  • Depth-resolved measurements were successfully performed.
  • Demonstrated capability for in vivo human skin analysis.

Conclusions:

  • The hybrid CRS-OCT system allows for efficient, targeted biochemical analysis of tissues.
  • This technology holds potential for in vivo applications such as tumor identification and margin assessment.
  • The system combines morphological screening with detailed biochemical confirmation.