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Conductive Covalent Organic Frameworks with Conductivity- and Pre-Reduction-Enhanced Electrochemiluminescence for
Jin-Ling Zhang1, Li-Ying Yao1, Yang Yang1
1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Analytical Chemistry
|February 14, 2022
Summary
This study introduces a conductive covalent organic framework (COF) for enhanced electrochemiluminescence (ECL) applications. The novel material significantly boosts ECL performance and enables ultrasensitive detection of thrombin in biosensors.
Area of Science:
- Materials Science
- Electrochemistry
- Nanotechnology
Background:
- Covalent organic frameworks (COFs) show promise in electrochemiluminescence (ECL) due to high luminophore loading and porous structures.
- The intrinsic poor conductivity of traditional COFs (<10-8 S m-1) limits their ECL performance.
- Enhancing COF conductivity is crucial for advancing ECL applications.
Purpose of the Study:
- To synthesize a conductive COF (HHTP-HATP-COF) to overcome the conductivity limitations of traditional COFs in ECL.
- To investigate the enhanced ECL performance of the conductive COF.
- To develop an ultrasensitive biosensor for thrombin detection using the novel COF material.
Main Methods:
- Synthesis of a conductive COF (HHTP-HATP-COF) using 2,3,6,7,10,11-hexaaminotriphenylene (HATP) and 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP).
- Characterization of the COF's conductivity (3.11 × 10-4 S m-1) and ECL properties.
- Development of an aptamer/protein proximity binding-induced DNA walker-based biosensor for thrombin detection utilizing the conductive COF as an ECL beacon.
Main Results:
- The synthesized HHTP-HATP-COF exhibited significantly improved conductivity compared to conventional COFs.
- The conductive COF demonstrated superior ECL performance, with intensity enhanced by pre-reduction electrolysis.
- The ECL beacon achieved ultrasensitive thrombin detection with a broad linearity (100 aM to 1 nM) and a low detection limit (62.1 aM).
Conclusions:
- The enhanced conductivity and pre-reduction strategy effectively boost COF-based ECL performance.
- Conductive COFs offer a promising platform for developing high-efficiency ECL materials.
- This work demonstrates the first application of conductive COFs in ECL biosensing, specifically for ultrasensitive thrombin detection.
