Celeribacter litoreus sp. nov., isolated from intertidal sediment

Area of Science:

• Microbiology
• Bacteriology
• Marine Biology

Background:

• Intertidal zones are crucial ecosystems harboring diverse microbial communities.
• The genus Celeribacter comprises marine bacteria with significant ecological roles.
• Characterization of novel bacterial species enhances our understanding of marine biodiversity.

Purpose of the Study:

• To isolate and characterize a novel bacterial strain from a coastal intertidal sediment.
• To determine the taxonomic placement of the isolated strain using polyphasic methods.
• To propose a new species within the Celeribacter genus.

Main Methods:

• Isolation and cultivation of bacteria from intertidal sediment.
• Physiological and biochemical characterization of the bacterial strain.
• Phylogenetic analysis based on 16S rRNA gene sequencing.
• Determination of genomic DNA G+C content, cellular fatty acid composition, polar lipids, and respiratory quinones.

Main Results:

• A Gram-negative, aerobic, rod-shaped bacterium, strain ASW11-22^T, was isolated.
• Strain ASW11-22^T exhibited optimal growth at 37°C, pH 7.0, and 1.0% NaCl.
• Phylogenetic analysis placed strain ASW11-22^T within the genus Celeribacter, with highest 16S rRNA gene similarity to Celeribacter halophilus (98.20%).
• Genomic DNA G+C content was 59.1 mol%, with major fatty acid being summed feature 8 and main polar lipids being phosphatidylglycerol and an unidentified aminolipid.

Conclusions:

• Strain ASW11-22^T represents a novel species within the genus Celeribacter.
• The proposed name for this new species is Celeribacter litoreus sp. nov.
• The type strain is ASW11-22^T (=KCTC 82495^T=MCCC 1K05584^T).