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Practical Method for Freezing Buck Semen.

Jane M Morrell1, Pongpreecha Malaluang1, Theodoros Ntallaris1

  • 1Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, SE-75007 Uppsala, Sweden.

Animals : an Open Access Journal From MDPI
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PubMed
Summary
This summary is machine-generated.

A simplified cryopreservation protocol for buck semen, using a soy lecithin extender and cold chain transport, achieved acceptable post-thaw motility and sperm quality. This method offers a practical solution for on-farm semen preservation.

Keywords:
chromatin integritygoat sperm cryopreservationplasma membrane integrityremoval of seminal plasmasoy lecithin semen extender

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Area of Science:

  • Reproductive Biology
  • Animal Science
  • Cryopreservation Technology

Background:

  • Existing buck semen cryopreservation protocols vary significantly, complicating selection for specific farm conditions.
  • Standardization of semen collection, extension, and sperm concentration is lacking across different cryopreservation methods.
  • On-farm cryopreservation presents logistical challenges due to transport and time constraints.

Purpose of the Study:

  • To develop and evaluate a simple, effective cryopreservation protocol for buck semen suitable for on-farm application.
  • To assess the post-thaw quality of buck semen preserved using a novel soy lecithin-based extender and cold chain transport.
  • To provide a practical and accessible method for long-term storage of buck genetic material.

Main Methods:

  • Semen was collected from seven bucks using an artificial vagina approximately 6 weeks before the breeding season.
  • Semen was immediately extended with a soy lecithin extender and maintained at a controlled temperature during transport (up to 4 hours).
  • Spermatozoa were concentrated, extended to 800 × 10^6 spermatozoa/mL, filled into 0.25 mL straws, and frozen above liquid nitrogen.

Main Results:

  • The developed protocol yielded acceptable post-thaw semen quality across all seven bucks.
  • Mean post-thaw sperm motility was 55 ± 21%, with a mean fragmented chromatin level of 3.27 ± 1.39%.
  • Normal sperm morphology exceeded 90% in all ejaculates, indicating high viability.

Conclusions:

  • The described simple cryopreservation protocol is effective for maintaining buck semen quality.
  • The soy lecithin extender and cold chain transport method are suitable for on-farm semen preservation and subsequent long-term storage.
  • This protocol offers a viable option for improving artificial insemination programs in goats through effective cryopreservation of buck semen.