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TFF1 in Aqueous Humor-A Potential New Biomarker for Retinoblastoma.
Maike Anna Busch1, André Haase1, Natalia Miroschnikov1
1Center for Translational Neuro- and Behavioral Sciences, Institute of Anatomy II, Department of Neuroanatomy, Medical Faculty, University of Duisburg-Essen, 45147 Essen, Germany.
Cancers
|February 15, 2022
Summary
Trefoil Factor Family Peptide 1 (TFF1) is detectable in the aqueous humor of retinoblastoma patients. This finding supports TFF1 as a promising new biomarker for childhood eye cancer diagnosis and treatment monitoring.
Area of Science:
- Ophthalmology
- Oncology
- Biochemistry
Background:
- Retinoblastoma (RB) is the most common pediatric eye cancer.
- Trefoil Factor Family Peptide 1 (TFF1) expression correlates with advanced RB stages.
- TFF1 is a potential biomarker for RB.
Purpose of the Study:
- To determine if TFF1 is detectable in the aqueous humor (AH) of RB patients.
- To evaluate TFF1 in AH as an accessible biomarker for RB diagnosis and therapy monitoring.
Main Methods:
- ELISA and Western blot analyses were used to investigate TFF1 expression.
- 15 retinoblastoma aqueous humor samples were analyzed.
- TFF1 levels were correlated with tumor origin and primary cell cultures.
Main Results:
- TFF1 was detected in 9 out of 15 (60%) aqueous humor samples.
- Detected TFF1 levels in AH correlated well with TFF1 levels in the original tumor.
- TFF1 expression in cell cultures generally reflected tumor levels, but secretion into AH varied.
Conclusions:
- TFF1 is a reliable biomarker detectable in the aqueous humor of retinoblastoma patients.
- TFF1 in AH offers a promising, accessible option for RB biomarker applications.
- Further research may elucidate the secretion dynamics of TFF1 in RB.
