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Related Experiment Videos

Ascorbate modulates antibacterial mechanisms in experimental pneumococcal pneumonia.

A L Esposito

    The American Review of Respiratory Disease
    |April 1, 1986
    PubMed
    Summary

    High doses of vitamin C (ascorbate) in mice enhanced lung clearance of Streptococcus pneumoniae at high bacterial loads but impaired it at low loads. Vitamin C altered pulmonary defenses without providing a clear host advantage.

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    An assessment of the factors contributing to the killing of type 3 Streptococcus pneumoniae by human polymorphonuclear leukocytes in vitro.

    APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·1990

    Area of Science:

    • Immunology
    • Microbiology
    • Nutritional Science

    Background:

    • Pulmonary antibacterial mechanisms are crucial for host defense against respiratory pathogens.
    • Vitamin C (ascorbate) is an essential nutrient with known immune-modulating properties.
    • The specific effects of high-dose vitamin C on lung immunity against Streptococcus pneumoniae remain incompletely understood.

    Purpose of the Study:

    • To investigate the impact of high-dose sodium ascorbate administration on the pulmonary antibacterial defenses against Streptococcus pneumoniae in a mouse model.
    • To assess the influence of vitamin C on bacterial clearance, immune cell recruitment, and macrophage function in the lungs.

    Main Methods:

    • Normal CD-1 mice were administered sodium ascorbate (200 mg/kg/24 h) or placebo.

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  • Mice were challenged intratracheally with varying inoculums of type 3 Streptococcus pneumoniae.
  • Survival rates, pulmonary bacterial clearance, granulocyte influx, and in vitro macrophage functions were evaluated.

    • Main Results:

    • Survival rates were similar between ascorbate-treated and control groups.
    • High inoculum challenge showed enhanced pneumococcal clearance and granulocyte influx in vitamin C-treated mice at 24 hours.
    • Lower inoculums revealed transient advantages in clearance and recruitment at 6 hours, while very low inoculums showed retarded clearance.
    • In vitro, vitamin C-treated macrophages exhibited reduced pneumococcidal capacity but maintained chemoattractant activity.

    Conclusions:

    • High doses of vitamin C significantly alter pulmonary defense mechanisms against Streptococcus pneumoniae in mice.
    • These alterations include dose-dependent effects on bacterial clearance and immune cell recruitment.
    • Despite observed changes in pulmonary defenses, high-dose vitamin C did not confer a substantial overall advantage to the host against pneumococcal infection.