[Microcalorimetric study of the oncogene EJ and proto-oncogene EC in the plasmid pBR 322]

Thermodynamic parameters were obtained of melting of the linear form of plasmids pBR 322 and plasmids pBR 322 with incorporated oncogene EC. The melting temperature of the main stage of heat absorption of the oncogene EJ was shown to be 1.6 degrees C lower than in the norm (EC). It is suggested that the thermostability change observed is related not only with point mutations of high-melting GC-pairs of bases for more low melting AT-pairs, but evidently with the structural changes in the DNA double helix as well.