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Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy
Jorge Berlanga-Acosta1, Maday Fernández-Mayola1, Yssel Mendoza-Marí1
1Tissue Repair, Wound Healing and Cytoprotection Research Group, Biomedical Research Direction, Center for Genetic Engineering and Biotechnology, Ave. 31 S/N. e/158 and 190, Cubanacán, Playa, Havana 10600, Cuba.
International Journal of Molecular Sciences
|February 15, 2022
Summary
Vascular disease can be transmitted via soluble messengers from human arterial tissue to rats, causing arterial wall thickening and cellular changes. This suggests a "vascular tissue memory" driving disease progression across species.
Area of Science:
- Vascular Biology
- Pathobiology
- Tissue Engineering
Background:
- Lower-extremity arterial disease (LEAD) is a significant health issue with rising prevalence, often resulting in amputation, disability, and mortality.
- The precise molecular mechanisms driving abnormal vascular wall remodeling in LEAD remain incompletely understood.
- A novel hypothesis suggests a transferable
Purpose of the Study:
- To investigate the existence and effects of a transferable
Main Methods:
- Arteriosclerotic human popliteal artery homogenates were infiltrated into rat wound granulation tissue for six days.
- Control groups received normal saline or healthy human brachial artery homogenates.
- Vascular morphology, endothelial cell phenotype, and immunohistochemical marker expression were analyzed.
Main Results:
- Infiltration of arteriosclerotic homogenates consistently induced significant arteriolar wall thickening in recipient rats.
- Recipient animals exhibited vascular morphological changes, including endothelial cell phenotypic reprogramming mirroring donor histopathology.
- Immunohistochemical marker expression patterns in rats matched the human donor tissue, demonstrating cross-species effects within days.
Conclusions:
- Soluble signaling messengers in arteriosclerotic tissue can induce vascular pathologies in recipient animals, suggesting a transferable
