Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:26

Membrane Fluidity

12.6K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
12.6K
Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

106.8K
Overview
106.8K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

8.2K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
8.2K
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

3.5K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Sphingosine-1-phosphate receptor 1/5 selective agonist alleviates ocular vascular pathologies.

Scientific reports·2026
Same author

Reduction in hippocampal cholinergic neurostimulating peptide enhances memory impairment in App<sup>NL-G-F</sup> KI mice.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Protective effects of a highly water-soluble rutin on cognitive dysfunction in mice.

Journal of pharmacological sciences·2026
Same author

Pulmonary edema following subarachnoid hemorrhage is associated with impairment of pulmonary vascular endothelial glycocalyx.

Biochemistry and biophysics reports·2026
Same author

Detrimental Effect of Early Clazosentan Administration on Neurogenic Pulmonary Edema Following Subarachnoid Hemorrhage and Safety of Its Delayed Administration: Laboratory Investigation.

World neurosurgery·2025
Same author

Macular white dot lesions with hyperreflective optical coherence tomography findings in cynomolgus and rhesus monkeys.

Molecular vision·2025

Related Experiment Video

Updated: Oct 3, 2025

Author Spotlight: Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies
06:16

Author Spotlight: Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies

Published on: July 28, 2023

2.8K

Lipid Droplet Accumulation Promotes RPE Dysfunction.

Tomohiro Yako1, Wataru Otsu2, Shinsuke Nakamura1

  • 1Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.

International Journal of Molecular Sciences
|February 15, 2022
PubMed
Summary
This summary is machine-generated.

Lipid droplet accumulation impairs retinal pigment epithelium (RPE) function in age-related macular degeneration (AMD). Inhibiting lipid droplet formation may offer a new therapeutic target for treating this progressive vision loss.

Keywords:
aginglipid dropletphagocytosisretinal pigment epithelium

More Related Videos

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.3K
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

19.9K

Related Experiment Videos

Last Updated: Oct 3, 2025

Author Spotlight: Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies
06:16

Author Spotlight: Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies

Published on: July 28, 2023

2.8K
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.3K
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

19.9K

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Molecular Biology

Background:

  • Non-exudative age-related macular degeneration (AMD) involves retinal pigment epithelium (RPE) dysfunction.
  • Impaired RPE phagocytosis is implicated in AMD progression, but mechanisms remain unclear.
  • Investigating the role of lipid droplets in RPE function is crucial for understanding AMD pathogenesis.

Purpose of the Study:

  • To investigate the effect of lipid droplet accumulation on RPE function.
  • To determine if inhibiting lipid droplet formation can ameliorate RPE dysfunction.

Main Methods:

  • Assessed lipid droplet-associated protein expression and lipid droplet presence in aged mice RPE-choroidal complex.
  • Induced lipid droplets in ARPE-19 cells in vitro using photoreceptor outer segments (POS) and oleic acid.
  • Evaluated the impact of lipid droplets on POS uptake and cell viability.
  • Utilized triacsin C to inhibit lipid droplet formation and assessed its effect on POS uptake and reactive oxygen species (ROS).

Main Results:

  • Aged mice showed increased lipid droplet-associated proteins and lipid droplets in RPE.
  • In vitro, lipid droplet formation in ARPE-19 cells reduced POS uptake without affecting cell viability.
  • Inhibiting lipid droplet formation with triacsin C improved POS uptake.
  • Triacsin C treatment also suppressed intracellular ROS levels.

Conclusions:

  • Lipid droplet accumulation is implicated in RPE dysfunction.
  • Inhibiting lipid droplet formation may be a potential therapeutic strategy for non-exudative AMD.
  • Targeting lipid droplet pathways could help prevent and treat AMD progression.