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Related Concept Videos

Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
Methods to Assess Microbial Communities01:19

Methods to Assess Microbial Communities

Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...

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Related Experiment Video

Updated: Jun 21, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
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The impacts of faecal subsampling on microbial compositional profiling.

Amanda J Cox1,2, Lily Hughes3, Tiffanie M Nelson4,5

  • 1School of Medical Science, Griffith Health Centre (G40), Griffith University, Parklands Drive, Southport, QLD, 4215, Australia. a.cox@griffith.edu.au.

BMC Research Notes
|February 15, 2022
PubMed
Summary
This summary is machine-generated.

Faecal subsampling from the beginning and end of a bowel movement shows consistent microbial profiles. This supports using small samples for large gut microbiome studies.

Keywords:
16s rRNAFaecal samplesGut microbiotaSub-sampling

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Area of Science:

  • Microbiology
  • Genomics
  • Human Health

Background:

  • Large-scale population studies increasingly use at-home, small-volume faecal collection kits.
  • Limited data exists on how faecal subsampling methods impact microbial compositional profiling.

Purpose of the Study:

  • To compare the microbial composition of faecal subsamples from the beginning and end of a single bowel movement.
  • To assess the reliability of small faecal samples for gut microbiome analysis.

Main Methods:

  • Ten healthy adults provided faecal subsamples (<5 g) from the start and end of a bowel movement.
  • Microbial composition was analyzed using V3-V4 16S rRNA sequencing.

Main Results:

  • No significant differences in operational taxonomic unit (OTU) count or Shannon diversity were found between subsamples.
  • Minor differences in the abundance of specific taxa (Bacillales, Eubacteriaceae, Eubacterium) were observed at lower taxonomic levels.
  • Overall, microbial compositional profiles were consistent between faecal subsamples.

Conclusions:

  • Faecal subsamples from different parts of a single bowel movement yield comparable microbial profiles.
  • This consistency supports the validity of using small faecal subsamples in large-scale gut microbiome studies.
  • The findings provide confidence in utilizing this approach for population-based faecal microbial profiling.