Fully resolved assembly of Cryptosporidium parvum
Vipin K Menon1, Pablo C Okhuysen2, Cynthia L Chappell3
1Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, USA.
Gigascience
|February 15, 2022
Summary
A high-quality Cryptosporidium parvum genome was assembled using Nanopore and Illumina sequencing. This complete reference genome aids in understanding parasite diversity and developing strategies against cryptosporidiosis.
Area of Science:
- Genomics
- Parasitology
- Infectious Diseases
Background:
- Cryptosporidium parvum is a widespread apicomplexan parasite with significant global human infection rates.
- Understanding parasite diversity and genomics is crucial for controlling infections and transmission.
- A complete and continuous reference genome is essential for robust genomic studies.
Purpose of the Study:
- To provide a highly accurate and complete reference genome for Cryptosporidium parvum.
- To detail methods for evaluating genome assembly approaches applicable to other Cryptosporidium species.
Main Methods:
- Genome assembly using Oxford Nanopore sequencing reads.
- Error correction and refinement with Illumina sequencing reads.
- Evaluation of assembly strategies for Cryptosporidium.
Main Results:
- A complete reference genome for Cryptosporidium parvum was generated.
- The assembly includes 8 chromosomes and 13 resolved telomeres.
- High completion rate achieved with 98.4% single-copy BUSCO genes.
Conclusions:
- The high-quality reference genome of a C. parvum subtype (IIaA17G2R1) is now available.
- This resource will facilitate comparative genomic studies within the Cryptosporidium clade.
- Enables enhanced understanding of parasite diversity, function, and host associations.
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