Related Experiment Video

Updated: Aug 26, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Homologous regions within M protein genes in group A streptococci of different serotypes

J R Scott, S K Hollingshead, V A Fischetti

    Infection and Immunity
    |May 1, 1986
    PubMed

    Insights

    The M6 protein

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Immunology

    Background:

    • The M protein is a major surface protein of Streptococcus pyogenes.
    • Understanding M protein variation is crucial for vaccine development and diagnostics.

    Purpose of the Study:

    • To investigate the structural conservation and variation of the M6 protein among different M serotypes.
    • To determine which regions of the M6 protein are conserved and which exhibit variability.

    Main Methods:

    • DNA hybridization using probes specific to the M6 protein gene.
    • Analysis of sequence conservation in the carboxy-terminal and amino-terminal regions.

    Main Results:

    • The carboxy-terminal region of the M6 protein is conserved across different M serotypes.
    • The amino-terminal region of the M6 protein shows significant variation among serotypes.

    Conclusions:

    • The conserved carboxy-terminal region may play a critical role in M6 protein function or structural integrity.
    • The variable amino-terminal region, accessible to the immune system, likely contributes to serotype diversity and immune evasion.

    More Related Videos

    Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)08:51

    Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

    Published on: November 4, 2016

    Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations08:03

    Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

    Published on: December 7, 2021

    Related Concept Videos

    Cross-reactivity00:42

    Cross-reactivity

    Overview
    Protein Families02:47

    Protein Families

    Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism.   Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members.   If these new proteins contain similar amino acids in key locations, protein...
    Cytoskeletal Proteins in Bacteria01:29

    Cytoskeletal Proteins in Bacteria

    Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
    Modern Molecular Taxonomy01:29

    Modern Molecular Taxonomy

    Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
    Bacterial Phylum Actinobacteria01:30

    Bacterial Phylum Actinobacteria

    Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
    Streptococcal Pharyngitis01:27

    Streptococcal Pharyngitis

    Streptococcal pharyngitis, commonly known as “strep throat,” is an acute infection of the oropharyngeal tissues caused by the Gram‑positive Group A Streptococcus (Streptococcus pyogenes). Transmission occurs primarily through respiratory droplets expelled during coughing, sneezing, or talking.Mechanisms of Host Entry and Immune EvasionUpon entering the host, S. pyogenes adheres to the mucosal epithelial cells of the pharynx via surface proteins, notably lipoteichoic acid and the antiphagocytic...