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Transcriptomic and functional analysis provides molecular insights into multicellular trichome development.

Mingming Dong1, Shudan Xue1, Ezra S Bartholomew1

  • 1Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China.

Plant Physiology
|February 16, 2022
PubMed
Summary

Researchers explored multicellular trichome development in cucumber plants, identifying key genes involved in forming both glandular and nonglandular trichomes. This study provides a valuable gene expression atlas for understanding plant hair development.

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Area of Science:

  • Plant Biology
  • Developmental Biology
  • Molecular Genetics

Background:

  • Trichomes are vital plant structures with diverse biological roles.
  • Unicellular trichome development is understood, but multicellular trichome formation remains less clear.
  • Understanding multicellular trichome development is crucial for plant science and agriculture.

Purpose of the Study:

  • To investigate the dynamic developmental processes of multicellular trichome formation in cucumber (Cucumis sativus L.).
  • To identify the molecular mechanisms underlying glandular and nonglandular multicellular trichome development.
  • To create a transcriptome atlas for cucumber multicellular trichome formation.

Main Methods:

  • Detailed morphological staging of multicellular trichome development.
  • Time-course RNA sequencing (RNA-seq) for transcriptome analysis.
  • Gene expression validation using reverse transcription-quantitative PCR (RT-qPCR) and in situ hybridization.
  • Virus-induced gene silencing (VIGS) to assess gene function.

Main Results:

  • Multicellular trichome development in cucumber was divided into five distinct stages.
  • A total of 711 multicellular trichome-related genes were identified.
  • Transcriptome analysis revealed key genes, including CsHOMEOBOX3 (CsHOX3) for nonglandular trichome elongation and CsbHLH1 for glandular trichome formation.
  • A model for multicellular trichome formation was proposed.

Conclusions:

  • This study provides a comprehensive transcriptome atlas for multicellular trichome formation in cucumber.
  • The findings offer insights into the molecular regulation of trichome development.
  • The identified genes and datasets serve as a crucial resource for future functional studies in plant trichome biology.