Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ia antigens in plastic-embedded tissues: a post-embedding immunohistochemical study.

W Hermanns, F Colbatzky, A Günther

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |June 1, 1986
    PubMed
    Summary

    This study developed a plastic embedding method for immunohistochemically detecting class II major histocompatibility complex (MHC) antigens (Ia antigens) in undecalcified rat joint tissues using glycol methacrylate (GMA). This technique preserves Ia antigenicity, enabling detailed subcellular analysis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Association Between Sleep Quality and Oxidative Stress in Postoperative Cardiovascular Patients-A Pilot Study.

    Journal of sleep research·2026
    Same author

    Diagnostic and prognostic implications of family history of fibrotic interstitial lung diseases.

    Respiratory research·2024
    Same author

    [Postoperative management following decompressive hemicraniectomy for malignant middle cerebral artery infarction-A German nationwide survey study].

    Der Nervenarzt·2023
    Same author

    Ultrasound-guided lymph node biopsy in smear-negative children and adolescents with suspected TB.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2023
    Same author

    [Refractory thrombotic thrombocytopenic purpura].

    Innere Medizin (Heidelberg, Germany)·2022
    Same author

    [Linguistic Validation of the "German Lung Fibrosis Health Related Quality of Life Questionnaire"].

    Pneumologie (Stuttgart, Germany)·2021

    Area of Science:

    • Immunology
    • Histology
    • Biochemistry

    Background:

    • Class II major histocompatibility complex (MHC) antigens, also known as Ia antigens, are crucial for immune responses.
    • Detecting Ia antigens in undecalcified joint tissues using immunohistochemistry presents technical challenges.
    • Preserving antigenicity during tissue processing is essential for accurate immunodetection.

    Purpose of the Study:

    • To establish an effective plastic embedding technique for immunohistochemical demonstration of Ia antigens in undecalcified joint tissues.
    • To optimize fixatives and dehydrating agents for preserving Ia immunoreactivity.
    • To compare post-embedding immunostaining with cryostat sections.

    Main Methods:

    • Testing various fixatives and dehydrating agents for embedding rat joint tissues in glycol methacrylate (GMA).

    Related Experiment Videos

  • Utilizing post-embedding immunostaining of thin (2-micron) sections.
  • Employing indirect immunoperoxidase and avidin-biotin complex (ABC) techniques.
  • Comparing results with cryostat sections.

    • Main Results:

    • Glycol methacrylate (GMA) dehydration, combined with 4% formaldehyde fixation, provided the best preservation of Ia antigenicity.
    • Ethylene glycol dehydration also showed good preservation of Ia immunoreactivity.
    • Thin sections allowed visualization of Ia antigens in distinct subcellular compartments and cell populations.

    Conclusions:

    • A robust plastic embedding technique using GMA is established for Ia antigen detection in undecalcified joint tissues.
    • The optimized method enhances the ability to study Ia antigen distribution at a subcellular level.
    • This technique facilitates detailed analysis of immune cell interactions within joint tissues.