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Versatile Bilayer Hydrogel for Wound Dressing through PET-RAFT Polymerization.

Jianzhi Liu1, Junkui Miao2, Ling Zhao2

  • 1State Key Laboratory of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

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A novel bilayer hydrogel dressing offers advanced wound healing by combining antibacterial inner layers with a tough outer layer. This multifunctional material accelerates healing, particularly for infected traumas.

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Wound Healing Research

Background:

  • Multifunctional hydrogels are promising for wound healing but difficult to create in a single structure.
  • Existing wound dressings often lack a combination of essential properties like antibacterial activity, mechanical strength, and biocompatibility.

Purpose of the Study:

  • To develop a bilayer hydrogel wound dressing with distinct functionalities in each layer.
  • To investigate the antibacterial, mechanical, and biocompatibility properties of the novel hydrogel.
  • To evaluate the efficacy of the bilayer hydrogel in accelerating wound healing in vivo.

Main Methods:

  • Fabrication of a bilayer hydrogel using N-isopropylacrylamide, chitosan-N-2-hydroxypropyl trimethylammonium chloride (HACC), polyvinyl alcohols, and acrylamide.
  • Covalent connection of hydrogel layers via photoinduced electron/energy transfer-reversible addition-fragmentation chain transfer (PET-RAFT) polymerization.
  • Assessment of antibacterial activity against Staphylococcus aureus and Escherichia coli, mechanical properties, biocompatibility (cytotoxicity assays), and in vivo wound healing efficacy in a full-thickness skin defect model.

Main Results:

  • The inner layer (Hm-PNn) exhibited softness, thermoresponsivity, excellent antibacterial activity against Gram-positive and Gram-negative bacteria, and high cell viability (>80%).
  • The outer layer (PVAo-PAmp) demonstrated good stretchability and toughness.
  • The bilayer hydrogel significantly accelerated wound healing, evidenced by improved collagen deposition and granulation tissue thickness in vivo.

Conclusions:

  • The developed bilayer hydrogel dressing effectively integrates multiple desirable functions.
  • The Hm-PNn/PVAo-PAmp hydrogel shows significant potential as an advanced wound dressing for accelerating healing, especially for open and infected wounds.