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Optothermophoretic flipping method for biomolecule interaction enhancement.

Jiajie Chen1, Youjun Zeng1, Jie Zhou1

  • 1Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, 518060, China.

Biosensors & Bioelectronics
|February 16, 2022
PubMed
Summary
This summary is machine-generated.

We developed optothermophoretic flipping (OTF), an active optical method to enhance biomolecule interactions. This technique significantly boosts sensitivity in biosensing by overcoming limitations of passive Brownian diffusion, enabling more efficient analysis.

Keywords:
Molecular interactionMolecule manipulationOptical manipulationOptofluidicsSurface plasmon resonance sensing

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Area of Science:

  • Biophysics
  • Biochemistry
  • Microfluidics

Background:

  • Surface-based biomolecule sensing is crucial for studying protein-protein interactions in microfluidic systems.
  • Current methods rely on passive Brownian diffusion, limiting sensing time and sensitivity, especially for low concentrations or single molecules.

Purpose of the Study:

  • To introduce an active, all-optical method, optothermophoretic flipping (OTF), to overcome limitations of passive diffusion in biosensing.
  • To demonstrate OTF's efficacy in enhancing biomolecule interactions and improving sensing performance.

Main Methods:

  • Developed optothermophoretic flipping (OTF), a novel temporal modulated technique.
  • Utilized flipped thermophoresis to actively enrich and bring biomolecules into contact with their counterparts.
  • Validated the method using antibody-antigen binding on a surface plasmon resonance imaging (SPRi) platform.

Main Results:

  • Achieved a 23.6-fold increase in sensitivity for biomolecule interaction sensing compared to Brownian diffusion alone.
  • Demonstrated OTF as an effective strategy for active biomolecule enrichment and interaction.
  • Showcased the method's potential for high-sensitivity applications in various biosensing platforms.

Conclusions:

  • Optothermophoretic flipping (OTF) offers a significant advancement over passive diffusion-based biosensing.
  • This active optical method enhances sensitivity and efficiency, particularly for low-concentration and single-molecule analyses.
  • OTF presents new possibilities for high-sensitivity biosensing in colloidal sciences and biochemical studies.