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Regeneration of T lymphocytes from human bone marrow cells after depletion with methylprednisolone
Abstract:
This study evaluates the propagation of T lymphocytes in bone marrow cells after in vitro methylprednisolone treatment. Previous studies from this laboratory showed that immunocompetent T lymphocytes could be eliminated from the bone marrow cells by incubation with 15 mg/ml (0.04 M) of methylprednisolone for 1 hr. The effectiveness of the chemoseparation was assessed by E-rosette forming cell assay, mitogen-induced lymphoblastogenic responses, and lymphocyte surface markers. In this study, bone marrow cells treated with different concentrations of methylprednisolone were cultured in the presence of PHA and Interleukin-2. The mitogen-induced lymphoblastogenesis was restored in the 15 mg/ml MP-treated bone marrow cells by the 8th day of culture in the presence of Interleukin-2. The cells in the culture were analyzed by E-rosette forming cell assay. It was shown that the predominant cells in the cultures were E-rosette forming cells. This study demonstrates that immunocompetent T lymphocytes can be regenerated in the presence of mitogen and Interleukin-2 from bone marrow cells treated with 15 mg/ml of methylprednisolone.
Insights
Methylprednisolone treatment eliminates T lymphocytes from bone marrow. However, immunocompetent T lymphocytes regenerate in culture with Interleukin-2 and PHA, demonstrating successful T cell regeneration.
Area of Science:
- Immunology
- Cell Biology
- Hematology
Background:
- Previous research demonstrated methylprednisolone's ability to eliminate T lymphocytes from bone marrow.
- Assessing chemoseparation effectiveness involved E-rosette assays, mitogen responses, and surface markers.
Purpose of the Study:
- To evaluate T lymphocyte propagation in bone marrow cells post-methylprednisolone treatment.
- To investigate the regeneration of immunocompetent T lymphocytes under specific culture conditions.
Main Methods:
- Bone marrow cells were treated with varying methylprednisolone concentrations.
- Cells were cultured with phytohemagglutinin (PHA) and Interleukin-2.
- E-rosette forming cell assay and mitogen-induced lymphoblastogenesis were used for analysis.
Main Results:
- Mitogen-induced lymphoblastogenesis was restored by day 8 in cells treated with 15 mg/ml methylprednisolone and cultured with Interleukin-2.
- E-rosette forming cells became predominant in these cultures.
- Demonstrated regeneration of immunocompetent T lymphocytes.
Conclusions:
- Immunocompetent T lymphocytes can regenerate from methylprednisolone-treated bone marrow cells.
- Interleukin-2 and mitogen presence are crucial for T lymphocyte regeneration.
- This finding has implications for T cell-based therapies and bone marrow transplantation.