Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Oct 3, 2025

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Published on: July 16, 2013

16.4K

Mechanical Stress Modulates Calcium-Activated-Chloride Currents in Differentiating Lens Cells.

Lisa Ebihara1,2, Pooja Acharya1, Jun-Jie Tong1

  • 1Center of Proteomics and Molecular Therapeutics, Rosalind Franklin University of Medicine and Science, North Chicago, IL, United States.

Frontiers in Physiology
|February 17, 2022
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

3.5K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.5K
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

6.9K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
6.9K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.8K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.8K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.4K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HuR-dependent expression of RyR2 contributes to calcium-mediated thermogenesis in murine brown adipocytes.

Scientific reports·2026
Same author

Establishment of <i>Galleria mellonella</i> as a model for <i>Achromobacter xylosoxidans</i> infection.

Frontiers in microbiology·2026
Same author

<i>Achromobacter xylosoxidans</i> isolates exhibit genome diversity, variable virulence, high levels of antibiotic resistance, and potential intrahost evolution.

mSphere·2026
Same author

Whole-genome sequences of <i>Achromobacter xylosoxidans</i> NIH-016 isolates - a set of multidrug-resistant strains collected longitudinally from the same patient.

Microbiology resource announcements·2025
Same author

<i>Achromobacter xylosoxidans</i> isolates exhibit genome diversity, variable virulence, high levels of antibiotic resistance and potential intrahost evolution.

bioRxiv : the preprint server for biology·2025
Same author

HuR inhibition reduces post-ischemic cardiac remodeling by dampening myocyte-dependent inflammatory gene expression and the innate immune response.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025

Mechanical stress rapidly activates calcium-activated chloride channels (CaCCs) in lens cells, suggesting their role in eye accommodation and volume regulation. This finding highlights a novel mechanism for lens function.

Area of Science:

  • Ophthalmology
  • Cell Physiology
  • Biophysics

Background:

  • The eye lens changes shape for focusing (accommodation) via ciliary muscle action.
  • Cellular fluid shifts within lens cells may accompany these shape changes.
  • Pressure-activated ion channels could regulate this cellular fluid flow.

Purpose of the Study:

  • To investigate if calcium-activated chloride channels (CaCCs) in lens cells are activated by mechanical stimulation.
  • To determine the role of CaCCs in lens accommodation and volume regulation.

Main Methods:

  • Whole-cell patch clamp technique applied to differentiating lens cells.
  • Mechanical stimulation induced by focal perfusion of individual lens cells.
  • Inhibition studies using TMEM16A-specific CaCC blocker Ani9 and calcium-depleted bath solution.
Keywords:
TMEM16Aaccommodationanoctamin-1chloridelensmechanosensitive channel

More Related Videos

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

1.0K
Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses
07:56

Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses

Published on: May 3, 2016

7.4K

Related Experiment Videos

Last Updated: Oct 3, 2025

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Published on: July 16, 2013

16.4K
Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

1.0K
Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses
07:56

Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses

Published on: May 3, 2016

7.4K

Main Results:

  • Focal perfusion caused a rapid, reversible increase in chloride current, indicating flow-induced channel activation.
  • The activation and recovery kinetics matched the timescale of accommodation.
  • The current was primarily mediated by TMEM16A chloride channels and did not require calcium influx.
  • Mechanical stress directly modulated CaCCs, independent of other mechanosensitive channels.

Conclusions:

  • Lens CaCCs are rapidly and reversibly modulated by mechanical stress.
  • These findings support the involvement of CaCCs in regulating lens cell volume during accommodation.
  • TMEM16A channels are key players in the mechanical response of lens cells.