Hyperspectral imaging for small-scale analysis of Hordeum vulgare L. leaves under the benzo[a]pyrene effect

Hyperspectral imaging is a newly developed approach to estimate the current state of the plants and to develop the methods of soil and plant ecological state improvement under the effect of different sources. The study was devoted to the novel approach of hyperspectral imaging application in the case of persistent organic pollutants (POP) uptake by plants. Hordeum vulgare L. was used as a test plant and grown on the soil artificially contaminated by benzo[a]pyrene (BaP) in the doses of 20, 100, 200, 400, and 800 ng g^-1, which corresponds to 1, 5, 10, 20, and 40 maximum permissible concentrations (MPC) and correlates with the level of soil pollution near industrial facilities in the Rostov Region (Russian Federation). It was analyzed a group of indexes responsible for plants stress, consists of broadband greenness group, narrowband greenness group, light use efficiency group, and leaf pigments group. Benzo[a]pyrene had a stronger effect on the efficiency of the photosynthesis process than on the content of chlorophylls. In the phase of active adaptation to stress in H. vulgare, the content of photosynthetic pigments was increased. The proposed method for selecting spectral profiles by cutting off profiles that do not belong to a plant, based on the NDVI value can be effectively used for the estimation of the plants stress under the BaP contamination and for future perspectives in the most suitable way for the application of the plant's growth stimulants.