Fast assay to predict multipotent mesenchymal stromal cell replicative senescence dynamics.

Area of Science:

• Regenerative Medicine
• Cell Biology
• Biotechnology

Background:

• Mesenchymal stromal cells (MSCs) are crucial for regenerative medicine.
• Large-scale *in vitro* expansion of MSCs is required but limited by replicative senescence.
• Cellular senescence reduces the therapeutic efficacy of MSCs, necessitating quality control.

Purpose of the Study:

• To develop a rapid assay to predict replicative senescence in adipose-derived stromal cells (ASCs).
• To establish a method for standardizing therapeutic MSCs before clinical application.

Main Methods:

• Developed a senescence evaluation assay using senescence inducers mitoxantrone (Mxt) and trichostatin A (TSA).
• Tested the assay on adipose-derived mesenchymal stromal cells (ASCs).
• Correlated induced senescence with spontaneous replicative senescence using Mxt.

Main Results:

• The assay successfully predicted the onset of spontaneous replicative senescence in ASCs.
• A strong correlation was confirmed between Mxt-induced senescence and spontaneous senescence.
• The developed protocol offers a fast and simple method for senescence evaluation.

Conclusions:

• The novel assay facilitates the prediction of MSC replicative senescence.
• This method aids in standardizing therapeutic ASCs and MSCs from various sources.
• Ensures the quality and efficacy of MSCs for regenerative medicine applications.