Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biosynthesis of macrophage activation factor.

B S Baliga, S D Rorher, A E Maumenee

    Lymphokine Research
    |January 1, 1986
    PubMed
    Summary

    Iron is essential for lymphokine-activated macrophage tumoricidal activity. Specific lymphokine binding to macrophages triggers protein synthesis, leading to tumor cell killing, but this process is impaired by iron deficiency.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Micronutrient levels in the cord blood of term neonates and its association with oxidative stress and neonatal anthropometry: a cross sectional study.

    La Clinica terapeutica·2026
    Same author

    Histology of Convergent Probing Appendages in Mormyridae.

    Integrative organismal biology (Oxford, England)·2023
    Same author

    Comparison of urine albumin creatinine ratio with the pediatric index of mortality 2 score for prediction of pediatric intensive care unit outcomes.

    Irish journal of medical science·2021
    Same author

    Comparative validity of microalbuminuria versus clinical mortality scores to predict pediatric intensive care unit outcomes.

    Clinical and experimental pediatrics·2019
    Same author

    Personal opinions on IDU and management of herpetic keratitis in its three different stages: epithelial, non-ulcerative stromal (metaherpetic) and chronic, ulcerative stromal.

    Highlights of ophthalmology·2014
    Same author

    Longwave-IR optical parametric oscillator in orientation-patterned GaAs pumped by a 2 µm Tm,Ho:YLF laser.

    Optics express·2013

    Area of Science:

    • Immunology
    • Molecular Biology

    Background:

    • Splenic lymphocytes from BCG-immunized mice produce proteins that activate macrophages.
    • These activated macrophages exhibit cytotoxic activity against tumor cells.

    Purpose of the Study:

    • To investigate the role of iron in lymphokine-dependent macrophage cytotoxic activity.
    • To elucidate the mechanism of lymphokine-induced tumoricidal activity in macrophages.

    Main Methods:

    • Culture of splenic lymphocytes with PPD and isolation of secreted proteins.
    • Assay of macrophage stimulating activity of protein fractions (50K-67K MW).
    • Analysis of lymphokine binding to macrophages and induced protein synthesis.

    Main Results:

    • A protein fraction between 50K-67K MW showed significant macrophage stimulating activity.
    • Iron deficiency in lymphocyte cultures reduced lymphokine activity.
    • Lymphokine specifically binds to macrophages, inducing protein synthesis (major peak at 55K) and tumoricidal activity.

    Conclusions:

    • Iron is crucial for lymphokine-induced macrophage tumoricidal activity.
    • Lymphokine binding to macrophages initiates a process involving protein synthesis.
    • This interaction is specific, saturable, and reversible, highlighting a key mechanism in immune response.

    Related Experiment Videos