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Assembly and Annotation of Escherichia coli Bacteriophage U115

William An1, Camilla Emsbo1, Emma Frey1

  • 1Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, USA.

Microbiology Resource Announcements
|February 17, 2022
PubMed

Insights

We sequenced the genome of Escherichia coli bacteriophage U115, a T4-like virus. This analysis identified 286 genes within its 166,986 base pair genome.

Area of Science:

  • Microbiology
  • Genomics
  • Virology

Background:

  • Bacteriophages are viruses that infect bacteria.
  • Escherichia coli bacteriophages are extensively studied due to their medical and industrial relevance.
  • T4-like bacteriophages represent a significant group with complex infection mechanisms.

Purpose of the Study:

  • To provide the first annotated genome sequence of Escherichia coli bacteriophage U115.
  • To characterize the genomic features of this T4-like bacteriophage.
  • To establish a foundational resource for future research on phage U115.

Main Methods:

  • Whole-genome sequencing of bacteriophage U115.
  • Bioinformatic analysis for gene prediction and annotation.
  • Comparative genomics to classify U115 within the T4-like phage family.

Main Results:

  • The complete genome sequence of bacteriophage U115 was determined to be 166,986 base pairs.
  • A total of 286 protein-coding genes were predicted and annotated.
  • Phage U115 was confirmed as a member of the T4-like bacteriophage group.

Conclusions:

  • The annotated genome sequence of bacteriophage U115 provides a comprehensive genetic blueprint.
  • This genomic data facilitates further investigation into the biology and evolution of T4-like phages.
  • Phage U115 represents a valuable model for studying bacterial-phage interactions in Escherichia coli.

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