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Related Experiment Video

Updated: Oct 3, 2025

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Published on: October 4, 2013

Intraductal Injections into the Mouse Mammary Gland.

Erik Oliemuller¹, Richard Newman¹, Beatrice A Howard²

¹The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|February 17, 2022

Summary

This summary is machine-generated.

The mammary intraductal xenografting technique allows direct injection of cells into mouse mammary ducts. This method better models preinvasive breast cancer progression to invasive disease by mimicking the natural microenvironment.

Keywords:

Intraductal mammary injection Preclinical model Xenograft

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Last Updated: Oct 3, 2025

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

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Published on: October 4, 2013

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In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

Published on: February 10, 2023

Area of Science:

Oncology
Cancer Research
Animal Models

Background:

The mammary fat pad xenografting method bypasses early invasive transitions.
Studying ductal carcinoma in situ (DCIS) progression requires a model that mimics the normal microenvironment.

Purpose of the Study:

To present a refined mammary intraductal xenografting technique.
To enable more relevant modeling of breast cancer progression from DCIS to invasive disease.

Main Methods:

Injecting cells or substances directly into the mammary ducts of female mice.
Utilizing either established breast cancer cell lines or patient-derived cells.
Detailed protocol procedures for performing the technique.

Main Results:

The technique allows for the injection of cells or substances directly into the mammary ducts.
This method successfully mimics the microenvironment of preinvasive breast lesions like DCIS.
Provides a more relevant model for studying the transition from DCIS to invasive breast cancer compared to fat pad injections.

Conclusions:

Mammary intraductal xenografting is a valuable technique for studying breast cancer progression.
This method offers a more accurate preclinical model for breast cancer research.
The protocol facilitates research into the early stages of breast cancer development.