Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid repetitive microinjection.

A M Colin

    Methods in Cell Biology
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    This study presents efficient methods for injecting echinoid eggs and zygotes with globin mRNA and radioactive amino acids. These techniques allow for precise peptide synthesis analysis using simple equipment.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Activation of a late H2B histone gene in blastula-stage sea urchin embryos by an unusual enhancer element located 3' of the gene.

    Molecular and cellular biology·1990
    Same author

    Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes.

    Proceedings of the National Academy of Sciences of the United States of America·1988
    Same author

    Evidence for simultaneous derepression of messenger RNA and the guanine nucleotide exchange factor in fertilized sea urchin eggs.

    Developmental biology·1987
    Same author

    Injected mRNA does not increase protein synthesis in unfertilized, fertilized, or ammonia-activated sea urchin eggs.

    Developmental biology·1986
    Same author

    [Antibiosis shown by a strain of Byssochlamys nivea Westling, 1909. III. Chromatographic characteristics and isolation of the active fraction].

    Mycopathologia·1975
    Same author

    Calcinosis universalis: report of two cases.

    Archives of physical medicine and rehabilitation·1967
    Same journal

    Quantification of cell viability by automated analysis of live cell imaging.

    Methods in cell biology·2026
    Same journal

    Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

    Methods in cell biology·2026
    Same journal

    Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

    Methods in cell biology·2026
    Same journal

    Screening and identification of protein-protein interaction using proximity labeling.

    Methods in cell biology·2026
    Same journal

    Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

    Methods in cell biology·2026
    Same journal

    Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

    Methods in cell biology·2026
    See all related articles

    Area of Science:

    • Developmental Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Echinoid eggs and zygotes are model systems for studying early development.
    • Accurate quantification of peptide synthesis is crucial for understanding cellular processes.

    Purpose of the Study:

    • To describe novel, efficient methods for injecting large numbers of echinoid cells.
    • To enable quantitative analysis of peptide synthesis following mRNA injection.

    Main Methods:

    • Injection of globin mRNA into echinoid eggs and zygotes.
    • Labeling of newly synthesized peptides with tritiated amino acids.
    • Quantitative analysis of peptide synthesis products.

    Main Results:

    Related Experiment Videos

  • Successful high-throughput injection of echinoid eggs and zygotes.
  • Quantitative data on peptide synthesis achievable.
  • Methods are rapid, cost-effective, and utilize simple equipment.

    • Conclusions:

    • The described injection methods are effective for large-scale experiments in echinoids.
    • These techniques are adaptable for injecting various molecules into diverse cell types.
    • Provides a valuable tool for molecular and developmental biology research.