Fast and sensitive graphene oxide-DNAzyme-based biosensor for Vibrio alginolyticus detection

Area of Science:

• Biotechnology
• Molecular Biology
• Environmental Science

Background:

• Pathogenic bacteria, such as Vibrio alginolyticus, pose significant public health risks.
• Rapid, cost-effective detection methods for bacterial pathogens are crucial but remain challenging.

Purpose of the Study:

• To select specific DNAzymes for Vibrio alginolyticus detection.
• To develop an enhanced DNAzyme-based sensor for rapid and sensitive bacterial detection.

Main Methods:

• Selection and activity assessment of DNAzymes using fluorescence and gel electrophoresis.
• Development of a graphene oxide (GO)-enhanced DNAzyme fluorescent sensor.
• Validation of the sensor using various seafood samples (crab, shrimp, fish, clam, oyster).

Main Results:

• Successfully selected Vibrio alginolyticus-specific DNAzymes, with DNAzyme DT1 showing a detection limit of 31 CFU/ml and high specificity.
• The GO-enhanced DNAzyme sensor significantly improved detection performance and reduced reaction time to 10 seconds.
• The method demonstrated effectiveness in detecting Vibrio alginolyticus in diverse seafood matrices.

Conclusions:

• A novel, rapid, and sensitive DNAzyme-based fluorescent sensor for Vibrio alginolyticus detection has been developed.
• The integration of graphene oxide enhances sensor performance and reaction speed.
• This method provides a promising tool for the reliable detection of Vibrio alginolyticus in food safety applications.