Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insulin binding in denervated muscle.

D Donaldson, O B Evans, R W Harrison

    Muscle & Nerve
    |March 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Denervation reduces insulin receptors in rat muscle by 40%, contributing to muscle atrophy. This study investigates the link between insulin receptor changes and muscle weight loss after denervation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sample preparation for actinide isotopic analysis using FIB-SEM.

    Journal of radioanalytical and nuclear chemistry·2026
    Same author

    Enhanced Radiation Tolerance of Tungsten Nanoparticles to He Ion Irradiation.

    Nanomaterials (Basel, Switzerland)·2018
    Same author

    Engineering self-organising helium bubble lattices in tungsten.

    Scientific reports·2017
    Same author

    An open-label, phase 2, single centre, randomized, crossover design bioequivalence study of AndroForte 5 testosterone cream and Testogel 1% testosterone gel in hypogonadal men: study LP101.

    Andrology·2016
    Same author

    Investigation of prognostic indicators for human uveal melanoma as biomarkers of canine uveal melanoma metastasis.

    The Journal of small animal practice·2014
    Same author

    Synopsis of Equine Veterinary Journal Supplement 37: Equine Ophthalmology III 'Reviews in memory of Keith Barnett'. Part 2.

    Equine veterinary journal·2011
    Same journal

    Join AANEM.

    Muscle & nerve·2026
    Same journal

    Targeted Muscle Reinnervation for Management and Prevention of Symptomatic Neuroma.

    Muscle & nerve·2026
    Same journal

    AANEM News & Insights.

    Muscle & nerve·2026
    Same journal

    Fat-Fraction Quantification Using Three-Point Dixon Technique in Duchenne Muscular Dystrophy and Its Correlation With Clinical Progression and Genotypic Characteristics: A Single Centre One-Year Prospective Study.

    Muscle & nerve·2026
    Same journal

    Characterizing Combined Central and Peripheral Demyelination-Insights From a Multimodal Comparison With Chronic Inflammatory Demyelinating Polyneuropathy and Multiple Sclerosis.

    Muscle & nerve·2026
    Same journal

    Electrical Modalities in the Rehabilitation of Peripheral Nerve Injuries: State of the Literature and Current Clinical Applications.

    Muscle & nerve·2026
    See all related articles

    Area of Science:

    • Endocrinology
    • Neuroscience
    • Muscle Physiology

    Background:

    • Muscle atrophy, or wasting, is a common consequence of nerve damage (denervation).
    • Insulin is a key hormone regulating muscle growth and metabolism.
    • The role of insulin receptors in denervation-induced muscle atrophy is not fully understood.

    Purpose of the Study:

    • To investigate changes in insulin receptor binding in rat muscle after denervation.
    • To determine if alterations in insulin receptor number or affinity correlate with muscle atrophy.

    Main Methods:

    • Insulin binding assays were performed on rat muscle tissue post-denervation.
    • Quantification of high-affinity insulin receptors and insulin binding dissociation constant (Kd) was conducted.
    • Muscle weight was measured to assess the degree of atrophy.

    Related Experiment Videos

    Main Results:

    • A significant 40% reduction in the number of high-affinity insulin receptors was observed.
    • No significant change in the insulin binding Kd was detected.
    • Affected muscles exhibited a 40%-60% reduction in weight, indicating substantial atrophy.

    Conclusions:

    • Denervation leads to a significant down-regulation of insulin receptors in rat muscle.
    • The decrease in insulin receptor number, not affinity, may play a role in the development of muscle atrophy.
    • These findings suggest a potential mechanism linking impaired insulin signaling to muscle wasting following denervation.