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RhoA, ROCK-1, and ROCK-2 Gene Expression and Polymorphisms in Cholesteatoma Patients
Kemal Görür1, Kansu Büyükafşar2, Etem Akbaş3
1Department of Otorhinolaryngology Faculty of Medicine, University of Mersin, Mersin, Turkey.
The Journal of International Advanced Otology
|February 18, 2022
Summary
Gene expression of Rho-A, ROCK-1, and ROCK-2 is reduced in cholesteatoma patients. The ROCK-2 AC/CC genotype may lower the risk of developing cholesteatoma.
Area of Science:
- Molecular Biology
- Genetics
- Otolaryngology
Background:
- Cholesteatoma is a skin condition in the middle ear.
- The roles of Rho-A, ROCK-1, and ROCK-2 in cholesteatoma are not well understood.
Purpose of the Study:
- To investigate gene polymorphism and expression of Rho-A, ROCK-1, and ROCK-2 in cholesteatoma patients.
Main Methods:
- Genotyping of ROCK-1, ROCK-2, and Rho-A gene polymorphisms using TaqMan SNP Genotyping Assays.
- Real-time polymerase chain reaction was used to determine gene expression levels.
- 120 cholesteatoma patients and 120 healthy controls were enrolled.
Main Results:
- Gene expression of Rho-A, ROCK-1, and ROCK-2 was significantly lower in cholesteatoma patients compared to controls.
- No significant difference in Rho-A and ROCK-1 gene polymorphisms between groups.
- ROCK-2 AC/CC genotype was found to be lower in cholesteatoma patients.
Conclusions:
- Reduced expression of Rho-A, ROCK-1, and ROCK-2 genes may be associated with cholesteatoma.
- The ROCK-2 C allele may confer a protective effect against cholesteatoma development.
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