Related Experiment Video
Updated: Oct 3, 2025
09:32
Generation of Myospheres From hESCs by Epigenetic Reprogramming
Published on: June 21, 2014
8.0K
CPEB1 directs muscle stem cell activation by reprogramming the translational landscape
Wenshu Zeng1,2,3,4,5, Lu Yue1,2,3,4,5, Kim S W Lam1,2,3,4,5
1Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, SAR, China.
Nature Communications
|February 18, 2022
Summary
Skeletal muscle stem cells (Satellite Cells or SCs) activate rapidly. This study reveals Cytoplasmic Polyadenylation Element Binding protein 1 (CPEB1) reprograms protein translation, driving SC activation and muscle regeneration.
Area of Science:
- Cell Biology
- Molecular Biology
- Developmental Biology
Background:
- Skeletal muscle stem cells (Satellite Cells, SCs) are quiescent but activate upon stimuli.
- Mechanisms governing rapid quiescent SC activation remain unclear.
Purpose of the Study:
- To elucidate the molecular mechanisms driving quiescent SC activation.
- To identify key regulators of the quiescence-to-activation transition in SCs.
Main Methods:
- Whole mouse perfusion fixation to isolate bona fide quiescent SCs.
- Proteomic and transcriptomic analyses to identify changes during SC activation.
- Investigated the role of Cytoplasmic Polyadenylation Element Binding protein 1 (CPEB1) in SC activation.
Main Results:
- Identified significant proteomic alterations in chromatin, metabolism, transcription, and translation during SC activation.
- Observed discordant transcriptomic and proteomic changes, suggesting translational regulation.
- Demonstrated CPEB1's role in post-transcriptional regulation of protein synthesis via 3' UTR binding.
- Showed CPEB1 promotes Myod1 synthesis, essential for SC activation and muscle regeneration.
Conclusions:
- CPEB1 is a critical regulator of the translational landscape during SC activation.
- CPEB1 controls SC activation and muscle regeneration by modulating protein synthesis.
- This work uncovers a key mechanism for rapid SC activation and muscle repair.
More Related Videos
07:18Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
Published on: April 19, 2018
21.9K
12:19Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA
Published on: February 12, 2020
8.0K
Related Concept Videos
01:58Cell Specific Gene Expression
14.1K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.1K
01:29Somatic to iPS Cell Reprogramming
2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K
02:23Master Transcription Regulators
7.2K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.2K
01:24Methods of Nuclear Reprogramming
1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
01:21Satellite Stem Cells and Muscular Dystrophy
2.1K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.1K
01:13Formation of Muscle Fibers from Myoblasts
5.3K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.3K