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Updated: Oct 3, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

scDART-seq: Mapping m⁶A at the single-cell level.

Huan Yao¹, Ying Yang¹, Yun-Gui Yang¹

¹CAS Key Laboratory of Genomic and Precision Medicine, Collaborative Innovation Center of Genetics and Development, College of Future Technology, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; China National Center for Bioinformation, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 101408, China.

|February 18, 2022

Summary

This summary is machine-generated.

Researchers developed single-cell DART-seq (scDART-seq) to map m6A RNA modifications in individual cells. This breakthrough illuminates the fundamental biology and regulatory roles of m6A at the single-cell level.

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Last Updated: Oct 3, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

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Published on: March 12, 2021

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

Area of Science:

Molecular Biology
Epigenetics
Single-cell Analysis

Background:

N6-methyladenosine (m6A) is a crucial epitranscriptomic mark regulating gene expression.
Understanding m6A dynamics in single cells is essential for deciphering cellular heterogeneity and function.
Previous methods lacked the resolution to profile m6A comprehensively at the single-cell level.

Purpose of the Study:

To establish a novel method for profiling m6A sites in single cells.
To investigate the fundamental biological features of m6A in individual cells.
To enhance the understanding of m6A's regulatory and functional mechanisms on a single-cell basis.

Main Methods:

Development and application of single-cell DART-seq (scDART-seq).
High-throughput sequencing for m6A site identification.
Computational analysis for characterizing m6A patterns in single cells.

Main Results:

Successful establishment of scDART-seq for precise m6A mapping in single cells.
Identification of distinct m6A modification patterns across different cell types or states.
Unveiling novel insights into the heterogeneity of m6A regulation within cell populations.

Conclusions:

scDART-seq provides unprecedented resolution for studying m6A epitranscriptomics.
The findings reveal fundamental principles of m6A biology at the single-cell level.
This work opens new avenues for exploring the role of m6A in cellular processes and disease.