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Upconversion-mediated CRISPR-Cas12a biosensing for sensitive detection of ochratoxin A
Zefeng Mao1, Xiaojuan Wang1, Ruipeng Chen1
1State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin, 300457, China; Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, China.
This study introduces a novel CRISPR/Cas12a biosensor for detecting ochratoxin A. The method utilizes an upconversion-magnetic probe for sensitive and rapid analysis, outperforming traditional kits.
Area of Science:
- Molecular Diagnostics
- Biosensing Technology
- CRISPR-Cas Systems
Background:
- CRISPR-Cas12a offers precise nucleic acid sequence identification for sensitive detection.
- Traditional fluorescent probes can be limiting in certain diagnostic applications.
Purpose of the Study:
- To develop a novel CRISPR/Cas12a-powered biosensing method for ochratoxin A detection.
- To replace conventional fluorescent probes with an upconversion-magnetic probe-DNA-Fe3O4 system.
Main Methods:
- Designed a biosensor utilizing CRISPR/Cas12a and nucleic acid aptamers.
- Employed an upconversion-magnetic probe-DNA-Fe3O4 probe for signal generation.
- Leveraged Cas protein activation chain and single-stranded DNA degradation activity.
Main Results:
- Achieved sensitive, rapid, and stable detection of ochratoxin A.
- The novel probe system successfully generated a fluorescent signal upon target capture.
- Demonstrated comparable detection capability to traditional commercial kits.
Conclusions:
- The developed CRISPR/Cas12a biosensor provides a rapid and sensitive method for ochratoxin A detection.
- The upconversion-magnetic probe offers a viable alternative to traditional fluorescent probes in diagnostics.
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