'Three-To-One' multi-functional nanocomposite-based lateral flow immunoassay for label-free and dual-readout...

Sandwich lateral flow immunoassays (LFIAs) based on paired antibodies are the most frequently used platform for food-borne pathogen detection. Although label-free strategies are used in LFIAs to avoid the utilization of paired antibodies, challenges of probe design and detection reliability still remain. Here, we report a new label-free and dual-readout LFIA (LD-LFIA) mediated by a 'Three-To-One' multi-functional nanocomposite with a unique combination of magnetic-adhesion-color-nanozyme properties. The strengths of the new designed nanocomposite are: (i) the Fe₃O₄ magnetic core simplifies the separation processes; (ii) surface adherent polydopamine (PDA) films exhibit a strong adhesion to pathogenic bacteria and provide colorimetric detection signal; and (iii) the deposited platinum nanoparticles (Pt NPs) can function as nanozymes to generate an extra catalytic signal for constructing a dual-readout mode to improve the detection accuracy. The resulting Fe₃O₄@PDA@Pt nanocomposite-based LD-LFIA can detect highly pathogenic Escherichia coli O157:H7 with limits of detection of 10² and 10 CFU mL^-1 for colorimetric and catalytic quantitative analyses, respectively. Systematic results also reveal that the proposed method exhibited high specificity and applicability for drinking water and chicken samples, serving as a promising tool for real bacterial sample testing. The multi-functional Fe₃O₄@PDA@Pt nanocomposite-based LD-LFIA can provide new ideas for designing new multi-functional probes for improving detection performance of conventional label-free LFIA and constructing more accurate and sensitive detection systems.