3D direct printing of composite bone scaffolds containing polylactic acid and spray dried mesoporous bioactive...

In this study, a three-dimensional composite scaffold is proposed consisting of polylactic acid and spray dried glass-ceramic microparticles (SGCMs). The compositional and structural characterization showed that the obtained spray dried powder formed as glass-ceramic (GC) with a completely interconnected porosity structure. Before direct printing of scaffolds, the rheological behavior of polylactic acid (PLA) and PLA-GC (PLA matrix containing SGCMs) inks were investigated. The PLA-GC composite ink represents sharper shear-thinning behavior and higher loss and storage modulus comparable to that of pure PLA. Microscopic observations and elemental mapping elements showed that 3D scaffolds had well-defined interconnected porosity and uniform distribution of the glass-ceramic particles. Mechanical tests indicated that compression strength is dependent on the scaffold porosity and the presence of SGCMs. Apatite formation evaluation besides ion release study showed better biomineralization capacity of PLA-GC scaffolds, as larger and denser sediments formed on the PLA-GC scaffolds after 7- and 14-day soaking. The preliminary cell response was studied with primary human mesenchymal stem cells (hMSCs) and revealed that SGCMs improved cell adhesion and viability and ALP activity. The appropriate combination of the biomaterials/methods to fabricate 3D porous constructs and their available bioactivity and biocompatibility, both being important characteristics for bone tissue engineering applications.